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DSPP present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1b
The plate has been pre-coated with Human CRKL antibody
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF11
Rat ET-2 (Endothelin 2) ELISA Kit Gp200 DSPP present in the sampleThe Rat (ET 2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rat samples. The plate has been pre coated with Rat ET 2 antibody. ET 2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ET 2 Antibody is added and binds to ET 2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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