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Substrate solution is then added and color develops in proportion to the amount of Rat S100B
Then Streptavidin-HRP is added and binds to the Biotinylated CTNND1 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL15
Then a biotinylated detection antibody specific for Mouse Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat SLC15A1 (Solute Carrier Family 15 Member 1) ELISA Kit 17Alpha Substrate solution is then addedThe Rat (SLC15A1) Solute Carrier Family 15 Member 1 ELISA Kit measures Solute carrier family 15 member 1 in Rat samples. The plate has been pre coated with Rat SLC15A1 antibody. SLC15A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SLC15A1 Antibody is added and binds to SLC15A1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SLC15A1 antibody. After incubation unbound
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