Rat IL-35 (Interleukin 35) ELISA Kit GMEM LOXL3 present in the sample

LOXL3 present in the sample is added and binds to antibodies coated on the wells

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GRN

Substrate solution is then added and color develops in proportion to the amount of Rat TGFB2

Then Streptavidin-HRP is added and binds to the Biotinylated NRG4 antibody

Rat IL-35 (Interleukin 35) ELISA Kit GMEM LOXL3 present in the sampleThe Rat IL 35 (Interleukin 35) ELISA Kit measures IL 35 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 35 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.