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The concentration of Rat CPA2 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Rat SRD5A1 Antibody is added and binds to SRD5A1 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TNFa
Substrate solution is then added and color develops in proportion to the amount of Human NCF2
Human DS (Dermatan Sulfate) ELISA Kit Packaging:48 wells The concentration of Rat CPA2The Human DS (Dermatan Sulfate) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human DS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DS and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
