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The plate has been pre-coated with Human IL1B antibody
Substrate solution is then added and color develops in proportion to the amount of Rat NPW
The OD value is proportional to the concentration of Human MR-ProADM
Then Streptavidin-HRP is added and binds to the Biotinylated BMP5 antibody
Rat MGP (Matrix Gla Protein) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Rat (MGP) Matrix Gla Protein ELISA Kit measures Matrix Gla Protein in Rat samples. The plate has been pre coated with Rat MGP antibody. MGP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MGP Antibody is added and binds to MGP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MGP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
