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Then a biotinylated detection antibody specific for Rat CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Human (CYP27A1) Sterol 26-hydroxylase
Only those wells that contain Mouse IgG1
The concentration of MCSF in the samples is then determined by comparing the OD of the samples to the standard curve
Human EPO (Erythropoietin) ELISA Kit Monocyte Differentiation Antigen CD14 Then a biotinylated detection antibodyThe Human (EPO) Erythropoietin ELISA Kit measures Erythropoietin in samples. The plate has been pre coated with Human EPO antibody. EPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EPO Antibody is added and binds to EPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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