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then it binds with Anti-FCER1A bound to precoated antibody
it binds with IL-9 bound to precoated antibody
Substrate solution is then added and color develops in proportion to the amount of Human CYP2C9
Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse GPC3 (Glypican 3) ELISA Kit Packaging:96 wells then it binds with Anti-FCER1AThe Mouse GPC3 (Glypican 3) ELISA Kit measures GPC3 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GPC3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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