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One aliquot is incubated at 0C (ice bath) and the other is incubated at 37C
You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Mouse SIRT6 antibody
The plate has been pre-coated with Mouse GRIN2A antibody
Porcine CKM (Creatine Kinase M-type) ELISA Kit Packaging:96 wells One aliquot is incubated atThe Porcine (CKM) Creatine Kinase M type ELISA Kit measures Creatine Kinase M Type in Porcine samples. The plate has been pre coated with Porcine CKM antibody. CKM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CKM Antibody is added and binds to CKM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CKM antibody. After incubation unbound Streptavidin HRP is washed away
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