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Substrate solution is then added and color develops in proportion to the amount of Human NPS
Substrate solution is then added and color develops in proportion to the amount of Mouse CISD2
Then Streptavidin-HRP is added and binds to the Biotinylated BCS1L antibody
Calculate the concentration of P4HA1 in the sample by plotting standard curve
Porcine NRP1 (Neuropilin 1) ELISA Kit HE Substrate solution is then addedThe Porcine NRP1 (Neuropilin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine NRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine NRP1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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