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Universal DHT in the sample or standard competes with a fixed amount of Universal DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHT
it binds with ECM29 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated KLK11 antibody
it binds with SIGLEC8 bound to precoated antibody
Mouse ANG-R-Tie1 (Angiopoietin Receptor Tie1) ELISA Kit Packaging:96 wells Universal DHT in the sampleThe Mouse ANG R Tie1 (Angiopoietin Receptor Tie1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse ANG R Tie1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG R Tie1 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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