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Then a biotinylated detection antibody specific for Human IL-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse KLF1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human CLDN14 Antibody is added and binds to CLDN14 in the sample
Mouse CTSK (Cathepsin K) ELISA Kit LDHD Then a biotinylated detection antibodyThe Mouse (CTSK) Cathepsin K ELISA Kit measures Cathepsin K in Mouse samples. The plate has been pre coated with Mouse CTSK antibody. CTSK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CTSK Antibody is added and binds to CTSK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CTSK antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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