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phenol-chloroform extraction
The concentration of Human IL4 in the samples is then determined by comparing the OD of the samples to the standard curve
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated
Recombinant Mouse Trefoil Factor 2 is produced by our Mammalian expression system and the target gene encoding Glu24-Tyr129 is expressed with a 6His tag at the C-terminus
Cor (Cortisol) ELISA Kit Packaging:48 wells phenol-chloroform extractionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Cor protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate
