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The concentration of Human FADS3 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPY1R
Substrate solution is then added and color develops in proportion to the amount of Rat SOD2
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Rat SIRT2 (Sirtuin 2) ELISA Kit LECT The concentration of Human FADS3The Rat (SIRT2) Sirtuin 2 ELISA Kit measures Sirtuin 2 in Rat samples. The plate has been pre coated with Rat SIRT2 antibody. SIRT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SIRT2 Antibody is added and binds to SIRT2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SIRT2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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