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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep E2 are added to the micro ELISA plate wells
The intensity of the color developed is inversely proportional to the concentration of PROG in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF13
And then biotinylated Human COMP Antibody is added and binds to COMP in the sample
Chicken COL2 (Collagen Type II) antibody ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Chicken COL2 (Collagen Type II) antibody ELISA Kit measures Anti COL2 in Chicken Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
