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NTN4 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human ANG-4 Antibody is added and binds to ANG-4 in the sample
Detects Gamma Hydroxybutyrate (GHB)
Substrate solution is then added and color develops in proportion to the amount of Human HTRA1
Mouse SFRP5 (Secreted Frizzled Related Protein 5) ELISA Kit PSF NTN4 present in the sampleThe Mouse (SFRP5) Secreted Frizzled Related Protein 5 ELISA Kit measures Secreted Frizzled Related Protein 5 in Mouse samples. The plate has been pre coated with Mouse SFRP5 antibody. SFRP5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SFRP5 Antibody is added and binds to SFRP5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SFRP5 antibody. After incubation unbound
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