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Then Streptavidin-HRP is added and binds to the Biotinylated CENPM antibody
only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color
And then biotinylated Mouse DKK3 Antibody is added and binds to DKK3 in the sample
Substrate solution is then added and color develops in proportion to the amount of Rat TF
Rabbit ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rabbit ECF CCL11 (Eosinophil Chemotactic Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit ECF CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit ECF CCL11 and Avidin Horseradish Peroxidase (HRP) conjugate are added
